AN UPDATE IN IMMUNOHISTOCHEMISTRY (PART I and II)

This 2-part special issue features 14 review articles with an attempt to cover IHC automation, standardization of diagnostic IHC, and the role of IHC in diagnosing tumors in major organs and tumors of unknown primary. This series begins with an article emphasizing standardization of diagnostic IHC in the preanalytic, analytic, and postanalytic phases, with a specific focus on (1) newly proposed guidelines on antibody validation from the College of American Pathologists Pathology and Laboratory Quality Center, (2) testing/optimizing a new antibody and troubleshooting, (3) interpreting and reporting IHC assay results, (4) continuing quality improvement programs, and (5) developing and implementing the concept of best practices in IHC.

from Fan Lin (2014) Evolving Practices of Diagnostic Immunohistochemistry. Archives of Pathology & Laboratory Medicine: December 2014, Vol. 138, No. 12, pp. 1561-1563.

PART I

Standardization of Diagnostic Immunohistochemistry: Literature Review and Geisinger Experience

Overview of Automated Immunohistochemistry

Immunohistochemistry in Undifferentiated Neoplasm/Tumor of Uncertain Origin

Utility of Immunohistochemistry in the Diagnosis of Pleuropulmonary and Mediastinal Cancers: A Review and Update

Application of Immunohistochemistry in Breast Pathology: A Review and Update

The Application of Immunohistochemical Biomarkers in Urologic Surgical Pathology

New Immunohistochemistry for B-Cell Lymphoma and Hodgkin Lymphoma

PART II

Application of Immunohistochemistry in Gastrointestinal and Liver Neoplasms: New Markers and Evolving Practice

Utility of Immunohistochemistry in the Pancreatobiliary Tract

The Utility of Immunohistochemistry in the Differential Diagnosis of Gynecologic Disorders

Review and Updates of Immunohistochemistry in Selected Salivary Gland and Head and Neck Tumors

Application of Immunohistochemistry in Thyroid Pathology

Immunohistochemistry in Dermatopathology

An Update on the Application of Newly Described Immunohistochemical Markers in Soft Tissue Pathology

Journal Club #9: Focal myositis, an intramuscular mass-like reactive process.

Focal myositis is an uncommon inflammatory pseudotumor of skeletal muscle that can be confused with a variety of neoplastic and inflammatory diseases. It is often misunderstood because it presents as a tumor-like mass, but histologically resembles a skeletal muscle myopathy or dystrophy. [...] Histologically, these were solitary intramuscular processes composed of variable myopathic and focal neurogenic changes, fibrosis, and inflammation, occasionally accompanied by prominent eosinophils.[...] Clinical diagnostic considerations ranged from benign entities such as rhabdomyoma, intramuscular lipoma, fibromatosis, myositis ossificans, proliferative myositis, inflammatory myofibroblastic tumor, and inflammatory myopathy to malignant entities such as rhabdomyosarcoma, leiomyosarcoma, liposarcoma, and lymphoma. Available follow-up revealed spontaneous regression.[...] Careful attention to reproducible clinicopathologic features can aid diagnosis and spare patients from excessive surgery or adverse therapy.

Auerbach A, et al. Focal myositis: a clinicopathologic study of 115 cases of an intramuscular mass-like reactive process. Am J Surg Pathol. 2009 Jul;33(7):1016-24.

Bibliography.

Layfield LJ, Crim J, Gupta D. Fine-needle aspiration findings in nodular myositis: a case report. Diagn Cytopathol. 2000 Nov;23(5):343-7

Le applicazioni della FISH in oncologia

Dopo l' interessante seminario, posto alcuni paper sull' argomento segnalati dal dr. Franco.

Wan TS, Ma ES. Molecular cytogenetics: an indispensable tool for cancer diagnosis. Chang Gung Med J. 2012 Mar-Apr;35(2):96-110.

Li Z, Li S, Bin Z, Zhang R, Li Y, Tian H, Chen W, Liu H. Potential clinical impact of three-dimensional visualization for fluorescent in situ hybridization image analysis. J Biomed Opt. 2012 May;17(5).

Ochs RC, Bagg A. Molecular genetic characterization of lymphoma: application to cytology diagnosis. Diagn Cytopathol. 2012 Jun;40(6):542-55.

Aukema SM, Siebert R, Schuuring E, van Imhoff GW, Kluin-Nelemans HC, Boerma EJ, Kluin PM. Double-hit B-cell lymphomas. Blood. 2011 Feb 24;117(8):2319-31. 

Szuhai K, Cleton-Jansen AM, Hogendoorn PC, Bovée JV. Molecular pathology and its diagnostic use in bone tumors. Cancer Genet. 2012 May;205(5):193-204.

Surgical Pathology Snapshot #3: a man with a painful slow growing lump of the thigh.

UOMO 54 ANNI

SEDE: coscia sinistra

NOTIZIE CLINICHE: tumefazione presente da 4 anni, che tuttavia da due è lentamente aumentata di volume, dolente alla palpazione.
MACRO: frammento giallastro di cm 2,5x1,5x0,7 centralmente sede di una lesione nodulare biancastra di cm 1,2.

(segnalato dal dr. S. Campione)

Surgical Pathology Snapshot #2: an elderly man with a 3 cm ulcerated scalp lesion.

Storia Clinica.

Lesione ulcerata del cuoio capelluto. di circa 3 cm presente da un pò di tempo anche se il paziente non sa dire con precisione da quanto: è un uomo di 76 aa,  con esiti da ustione a 18 mesi nella zona dove è insorta la lesione. IHC:  S100 -, CD34-, EMA-, CK-; Vim +...

What's your diagnosis?

(segnalato dalla dott.ssa J. Falleti)